https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31426 CBE1 expression in normal as well as impaired spermatogenesis on mRNA (quantitative reverse-transcription polymerase chain reaction and in situ hybridization) and protein level (immunohistochemistry, Western blot analysis). Result(s): In normal spermatogenesis, CBE1 mRNA was expressed in late pachytene spermatocytes, and the protein was localized within the flagellum of elongating spermatids from stage V up to the spermiation in stage II. Immunoelectron microscopy showed CBE1 clearly associated with microtubules at the manchette, the head-tail coupling apparatus, and the flagellum, but the protein was absent in spermatozoa. Compared with normal spermatogenesis, CBE1 mRNA was statistically significantly reduced in samples with a maturation arrest at the level of round spermatids and primary spermatocytes, and was absent in samples showing Sertoli cell-only syndrome. CBE1 protein was completely missing in SDA samples showing few elongating spermatids. Conclusion(s): Our data strongly suggest an influence of CBE1 in ciliogenesis in spermatids due to the localization at the microtubules of the elongating spermatids, indicating a role in the intramanchette and/or intraflagellar transport mechanism. The absence of CBE1 in spermatozoa suggests that CBE1 is important for the spermatid development but not for the maintenance of mature spermatozoa as a component of the flagellum.]]> Wed 11 Jul 2018 17:04:16 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1512 Sat 24 Mar 2018 08:30:26 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:199 Sat 24 Mar 2018 07:42:54 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24273 Sat 24 Mar 2018 07:14:56 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48643 Fri 24 Mar 2023 13:37:38 AEDT ]]>